Single-cell multimodal profiling reveals cellular epigenetic heterogeneity.
Document Type
Article
Publication Date
10-2016
JAX Source
Nat Methods 2016 Oct; 13(10):833-6
Volume
13
Issue
10
First Page
833
Last Page
836
ISSN
1548-7105
PMID
27525975
Abstract
Sample heterogeneity often masks DNA methylation signatures in subpopulations of cells. Here, we present a method to genotype single cells while simultaneously interrogating gene expression and DNA methylation at multiple loci. We used this targeted multimodal approach, implemented on an automated, high-throughput microfluidic platform, to assess primary lung adenocarcinomas and human fibroblasts undergoing reprogramming by profiling epigenetic variation among cell types identified through genotyping and transcriptional analysis. Nat Methods 2016 Oct; 13(10):833-6.
Recommended Citation
Cheow L,
Courtois E,
Tan Y,
Viswanathan R,
Xing Q,
Tan R,
Tan D,
Robson P,
Loh Y,
Quake S,
Burkholder W.
Single-cell multimodal profiling reveals cellular epigenetic heterogeneity. Nat Methods 2016 Oct; 13(10):833-6