Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics.
Document Type
Article
Publication Date
8-22-2017
JAX Source
Cell Rep 2017 Aug 22; 20(8):1978-1990
Volume
20
Issue
8
First Page
1978
Last Page
1990
ISSN
2211-1247
PMID
28834758
DOI
https://doi.org/10.1016/j.celrep.2017.08.003
Abstract
There is growing interest in using embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) derivatives for tissue regeneration. However, an increased understanding of human immune responses to stem cell-derived allografts is necessary for maintaining long-term graft persistence. To model this alloimmunity, humanized mice engrafted with human hematopoietic and immune cells could prove to be useful. In this study, an in-depth analysis of graft-infiltrating human lymphocytes and splenocytes revealed that humanized mice incompletely model human immune responses toward allogeneic stem cells and their derivatives. Furthermore, using an "allogenized" mouse model, we show the feasibility of reconstituting immunodeficient mice with a functional mouse immune system and describe a key role of innate immune cells in the rejection of mouse stem cell allografts. Cell Rep 2017 Aug 22; 20(8):1978-1990
Recommended Citation
Kooreman N,
de Almeida P,
Stack J,
Nelakanti R,
Diecke S,
Shao N,
Swijnenburg R,
Sanchez-Freire V,
Matsa E,
Liu C,
Connolly A,
Hamming J,
Quax P,
Brehm M,
Greiner D,
Shultz LD,
Wu J.
Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics. Cell Rep 2017 Aug 22; 20(8):1978-1990