QTL Mapping and Identification of Candidate Genes in DO Mice: A Use Case Model Derived from a Benzene Toxicity Experiment.

Document Type

Article

Publication Date

2017

JAX Location

Reprint Collection

JAX Source

Methods Mol Biol 2017; 1488:265-281

Volume

1488

First Page

265

Last Page

281

ISSN

1940-6029

PMID

27933529

DOI

https://doi.org/10.1007/978-1-4939-6427-7_12

Grant

GM076468, GM070683

Abstract

Diversity Outbred (DO) mice are a multiparental advanced generation intercross population derived from eight inbred strains which are genetically very diverse. They are maintained as an outbred population using a randomized mating design. Thus DO mice represent an ideal population to map phenotypic traits. Here, we provide a case study in which male DO mice were exposed to benzene and phenotyped for the number of micronucleated reticulocytes. We provide step-by-step R scripts for the analysis of phenotypes, genotypes, mapping of resistance gene loci and identification of candidate genes. Methods Mol Biol 2017; 1488:265-281.

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