Generating Mouse Models Using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput.
Document Type
Article
Publication Date
1-1-2017
Publication Title
Methods in molecular biology (Clifton, N.J.)
Keywords
JMG, Animals, CRISPR-Cas Systems, Electroporation, Endonucleases, Mice, Mice, Transgenic, Models, Animal, Zygote
JAX Source
Methods Mol Biol . 2017:1605:219-230
Volume
1605
First Page
219
Last Page
230
ISSN
1940-6029
PMID
28456968
DOI
10.1007/978-1-4939-6988-3_15
Abstract
Mouse models with genetic modifications are widely used in biology and biomedical research. Although the application of CRISPR-Cas9 system greatly accelerated the process of generating genetically modified mice, the delivery method depending on manual injection of the components into the embryos remains a bottleneck, as it is laborious, low throughput, and technically demanding. To overcome this limitation, we invented and optimized the ZEN (Zygote electroporation of nucleases) technology to deliver CRISPR-Cas9 reagents via electroporation. Using ZEN, we were able to generate genetically modified mouse models with high efficiency and throughput. Here, we describe the protocol in great detail.
Recommended Citation
Wang W,
Zhang Y,
Wang H.
Generating Mouse Models Using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput. Methods Mol Biol . 2017:1605:219-230