Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA).

Authors

Zijian Tang, The Jackson LaboratoryFollow
Chengkai Dai, The Jackson LaboratoryFollow

Document Type

Article

Publication Date

2017

JAX Location

Reprint Collection

JAX Source

Methods Mol Biol 2017; 1487:195-201.

Volume

1487

First Page

195

Last Page

201

ISSN

1940-6029

PMID

27924568

DOI

https://doi.org/10.1007/978-1-4939-6424-6_14

Abstract

RAS/MAPK signaling responds to diverse extracellular cues and regulates a wide array of cellular processes. Given its biological importance, abnormalities in RAS/MAPK signaling cascade have been intimately implicated in numerous human diseases, including cancer. Herein, we describe a novel methodology to study activation of this pivotal signaling pathway. The Proximity Ligation Assay (PLA) is employed to monitor kinase-substrate interactions between MEK1 and HSF1, or MEK1 and ERK1 in situ. Methods Mol Biol 2017; 1487:195-201.

Recommended Citation

Tang Z, Dai C. Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA). Methods Mol Biol 2017; 1487:195-201.