Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells.
Document Type
Article
Publication Date
5-2019
Keywords
JGM
JAX Source
Nat Methods 2019 May; 16(5):409-412
Volume
16
Issue
5
First Page
409
Last Page
412
ISSN
1548-7105
PMID
31011186
DOI
https://doi.org/10.1038/s41592-019-0392-0
Grant
CA034196,GM124998
Abstract
Multimodal single-cell assays provide high-resolution snapshots of complex cell populations, but are mostly limited to transcriptome plus an additional modality. Here, we describe expanded CRISPR-compatible cellular indexing of transcriptomes and epitopes by sequencing (ECCITE-seq) for the high-throughput characterization of at least five modalities of information from each single cell. We demonstrate application of ECCITE-seq to multimodal CRISPR screens with robust direct single-guide RNA capture and to clonotype-aware multimodal phenotyping of cancer samples.
Recommended Citation
Mimitou E,
Cheng A,
Montalbano A,
Hao S,
Stoeckius M,
Legut M,
Roush T,
Herrera A,
Papalexi E,
Ouyang Z,
Satija R,
Sanjana N,
Koralov S,
Smibert P.
Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells. Nat Methods 2019 May; 16(5):409-412