Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells.
Nat Methods 2019 May; 16(5):409-412
Multimodal single-cell assays provide high-resolution snapshots of complex cell populations, but are mostly limited to transcriptome plus an additional modality. Here, we describe expanded CRISPR-compatible cellular indexing of transcriptomes and epitopes by sequencing (ECCITE-seq) for the high-throughput characterization of at least five modalities of information from each single cell. We demonstrate application of ECCITE-seq to multimodal CRISPR screens with robust direct single-guide RNA capture and to clonotype-aware multimodal phenotyping of cancer samples.
Mimitou, Eleni P; Cheng, Anthony; Montalbano, Antonino; Hao, Stephanie; Stoeckius, Marlon; Legut, Mateusz; Roush, Timothy; Herrera, Alberto; Papalexi, Efthymia; Ouyang, Zhengqing; Satija, Rahul; Sanjana, Neville E; Koralov, Sergei B; and Smibert, Peter, "Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells." (2019). Faculty Research 2019. 109.