Title

Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells.

Document Type

Article

Publication Date

5-2019

Keywords

JGM

JAX Source

Nat Methods 2019 May; 16(5):409-412

PMID

31011186

DOI

https://doi.org/10.1038/s41592-019-0392-0

Grant

GM124998

Abstract

Multimodal single-cell assays provide high-resolution snapshots of complex cell populations, but are mostly limited to transcriptome plus an additional modality. Here, we describe expanded CRISPR-compatible cellular indexing of transcriptomes and epitopes by sequencing (ECCITE-seq) for the high-throughput characterization of at least five modalities of information from each single cell. We demonstrate application of ECCITE-seq to multimodal CRISPR screens with robust direct single-guide RNA capture and to clonotype-aware multimodal phenotyping of cancer samples.

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