Longitudinal profiling of human blood transcriptome in healthy and lupus pregnancy.
Document Type
Article
Publication Date
5-2019
Keywords
JGM
JAX Source
J Exp Med 2019 May; 216(5):1154-1169
Volume
216
Issue
5
First Page
1154
Last Page
1169
ISSN
1540-9538
PMID
30962246
DOI
https://doi.org/10.1084/jem.20190185
Grant
AR070594
Abstract
Systemic lupus erythematosus carries an increased risk of pregnancy complications, including preeclampsia and fetal adverse outcomes. To identify the underlying molecular mechanisms, we longitudinally profiled the blood transcriptome of 92 lupus patients and 43 healthy women during pregnancy and postpartum and performed multicolor flow cytometry in a subset of them. We also profiled 25 healthy women undergoing assisted reproductive technology to monitor transcriptional changes around embryo implantation. Sustained down-regulation of multiple immune signatures, including interferon and plasma cells, was observed during healthy pregnancy. These changes appeared early after embryo implantation and were mirrored in uncomplicated lupus pregnancies. Patients with preeclampsia displayed early up-regulation of neutrophil signatures that correlated with expansion of immature neutrophils. Lupus pregnancies with fetal complications carried the highest interferon and plasma cell signatures as well as activated CD4
Recommended Citation
Hong S,
Banchereau R,
Maslow B,
Guerra M,
Cardenas J,
Baisch J,
Branch D,
Porter T,
Sawitzke A,
Laskin C,
Buyon J,
Merrill J,
Sammaritano L,
Petri M,
Gatewood E,
Cepika A,
Ohouo M,
Obermoser G,
Anguiano E,
Kim T,
Nulsen J,
Nehar-Belaid D,
Blankenship D,
Turner J,
Banchereau J,
Salmon J,
Pascual V.
Longitudinal profiling of human blood transcriptome in healthy and lupus pregnancy. J Exp Med 2019 May; 216(5):1154-1169
Comments
Partially funded by the Kathryn W. Davis gift to The Jackson Laboratory.