IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8α
Document Type
Article
Publication Date
7-16-2019
Keywords
JGM
JAX Source
Immunity 2019 Jul 16; 51(1):64-76.e7
Volume
51
Issue
1
First Page
64
Last Page
76
ISSN
1097-4180
PMID
31231033
DOI
https://doi.org/10.1016/j.immuni.2019.05.011
Grant
AI133440
Abstract
Type 1 CD8α+ conventional dendritic cells (cDC1s) are required for CD8+ T cell priming but, paradoxically, promote splenic Listeria monocytogenes infection. Using mice with impaired cDC2 function, we ruled out a role for cDC2s in this process and instead discovered an interleukin-10 (IL-10)-dependent cellular crosstalk in the marginal zone (MZ) that promoted bacterial infection. Mice lacking the guanine nucleotide exchange factor DOCK8 or CD19 lost IL-10-producing MZ B cells and were resistant to Listeria. IL-10 increased intracellular Listeria in cDC1s indirectly by reducing inducible nitric oxide synthase expression early after infection and increasing intracellular Listeria in MZ metallophilic macrophages (MMMs). These MMMs trans-infected cDC1s, which, in turn, transported Listeria into the white pulp to prime CD8+ T cells. However, this also facilitated bacterial expansion. Therefore, IL-10-mediated crosstalk between B cells, macrophages, and cDC1s in the MZ promotes both Listeria infection and CD8+ T cell activation.
Recommended Citation
Liu D,
Yin X,
Olyha S,
Nascimento M,
Chen P,
White T,
Gowthaman U,
Zhang T,
Gertie J,
Zhang B,
Xu L,
Yurieva M,
Devine L,
Williams A,
Eisenbarth S.
IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8α Immunity 2019 Jul 16; 51(1):64-76.e7