Development and validation of a novel 29-plex Y-STR typing system for forensic application.

Document Type

Article

Publication Date

10-1-2019

Keywords

JGM

JAX Source

Forensic Sci Int Genet 2019 Oct 1; 44:102169

Volume

44

First Page

102169

Last Page

102169

ISSN

1878-0326

PMID

31614310

DOI

https://doi.org/10.1016/j.fsigen.2019.102169

Abstract

Short tandem repeat within the male-specific part of the human Y chromosome (Y-STR) is an effective forensic tool in mixture identification, patrilineal relationship evaluation, and familial searches. Despite their usefulness, current Y-STR-based genotyping systems often lack the discriminatory power to resolve genetic relationships between distant relatives or within patrilocal populations. In this study, we developed a novel Y-STR 29-plex typing system, which combined the 17 Y-STR loci used in the AmpFLSTR® Yfiler® PCR Amplification Kit (Yfiler), eight Y-STR loci with a low-medium mutation rate, and four rapidly mutating Y-STR loci. The system was generated to achieve greater discriminatory power between male subjects and improved ability to infer haplogroup classifications. The system was extensively tested on data from 752 individuals for its sensitivity, male specificity, species specificity, mixture resolution, reproducibility, concordance, stutter and size accuracy, precision, and population genetics, following the Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. The results demonstrated that the Y-STR 29-plex typing system was time-efficient, reproducible, accurate, sensitive, and robust to familial searching and paternal biogeographic ancestry inference.

Please contact the Joan Staats Library for information regarding this document.

Share

COinS