Document Type
Article
Publication Date
2-18-2019
Keywords
JMG
JAX Source
Commun Biol 2019 Feb 18; 2:70
Volume
2
First Page
70
Last Page
70
ISSN
2399-3642
PMID
30793048
DOI
https://doi.org/10.1038/s42003-019-0321-x
Abstract
A persistent concern with CRISPR-Cas9 gene editing has been the potential to generate mutations at off-target genomic sites. While CRISPR-engineering mice to delete a ~360 bp intronic enhancer, here we discovered a founder line that had marked immune dysregulation caused by a 24 kb tandem duplication of the sequence adjacent to the on-target deletion. Our results suggest unintended repair of on-target genomic cuts can cause pathogenic "bystander" mutations that escape detection by routine targeted genotyping assays.
Recommended Citation
Simeonov D,
Brandt A,
Chan A,
Cortez J,
Li Z,
Woo J,
Lee Y,
Carvalho C,
Indart A,
Roth T,
Zou J,
May A,
Lupski J,
Anderson M,
Buaas FW,
Rokhsar D,
Marson A.
A large CRISPR-induced bystander mutation causes immune dysregulation. Commun Biol 2019 Feb 18; 2:70
Comments
This open access article is licensed under a Creative Commons Attribution 4.0 International License