Uncoupling of transcriptomic and cytological differentiation in mouse spermatocytes with impaired meiosis.
Document Type
Article
Publication Date
3-1-2019
Keywords
JMG
JAX Location
Reprint Collection
JAX Source
Mol Biol Cell 2019 Mar 1; 30(5):717-728
Volume
30
Issue
5
First Page
717
Last Page
728
ISSN
1939-4586
PMID
30649999
DOI
https://doi.org/10.1091/mbc.E18-10-0681
Grant
GM099640, HD007065
Abstract
Cell differentiation is driven by changes in gene expression that manifest as changes in cellular phenotype or function. Altered cellular phenotypes, stemming from genetic mutations or other perturbations, are widely assumed to directly correspond to changes in the transcriptome and vice versa. Here, we exploited the cytologically well-defined Prdm9 mutant mouse as a model of developmental arrest to test whether parallel programs of cellular differentiation and gene expression are tightly coordinated, or can be disassociated. By comparing cytological phenotype markers and transcriptomes in wild-type and mutant spermatocytes, we identified multiple instances of cellular and molecular uncoupling in Prdm9
Recommended Citation
Fine A,
Ball R,
Fujiwara Y,
Handel M,
Carter GW.
Uncoupling of transcriptomic and cytological differentiation in mouse spermatocytes with impaired meiosis. Mol Biol Cell 2019 Mar 1; 30(5):717-728
Comments
We thank the Handel and Carter laboratories for discussions, and gratefully acknowledge Sabrina Petri for animal care. We also thank Drs. J. Trowbridge, A. Yee, C. Cowan, and S. Munger for feedback on the project and comments on the manuscript.