Creation of PDX-Bearing Humanized Mice to Study Immuno-oncology.
Document Type
Article
Publication Date
2019
JAX Source
Methods Mol Biol 2019; 1953:241-252
Volume
1953
First Page
241
Last Page
252
ISSN
1940-6029
PMID
30912026
DOI
https://doi.org/10.1007/978-1-4939-9145-7_15
Abstract
A significant obstacle to the study of human cancer biology and the testing of human specific immunotherapeutics is the paucity of translational models that recapitulate both the growth of human tumors and the functionality of human immune systems. Humanized mice engrafted with human hematopoietic stem cells (HSC) and patient-derived xenografts (PDX) enable preclinical investigation of the interactions between the human immune system and human cancer. We use immunodeficient non-obese diabetic (NOD, scid, gamma) NSG™ or NSG™-SGM3 mice as hosts for establishment of human immunity following HSC injection and for engraftment of human tumors. Here we describe a refined protocol for the subcutaneous implant of solid PDX tumors into humanized mice. Protocols to recover infiltrating immune cells from growing tumors and to evaluate the immune cell subsets by flow cytometry are also described.
Recommended Citation
Yao L,
Aryee K,
Cheng M,
Kaur P,
Keck JG,
Brehm M.
Creation of PDX-Bearing Humanized Mice to Study Immuno-oncology. Methods Mol Biol 2019; 1953:241-252
Comments
We thank Justin Jarvi and Marta Tewodros for trocar method development and technical assistance. This work was supported by The Jackson Laboratory.