Mapping systemic lupus erythematosus heterogeneity at the single-cell level.
Document Type
Article
Publication Date
9-2020
Keywords
JGM, JAXCC
JAX Source
Nat Immunol 2020 Sep; 21(9):1094-1106
Volume
21
Issue
9
First Page
1094
Last Page
1106
ISSN
1529-2916
PMID
32747814
DOI
https://doi.org/10.1038/s41590-020-0743-0
Grant
CA034196
Abstract
Patients with systemic lupus erythematosus (SLE) display a complex blood transcriptome whose cellular origin is poorly resolved. Using single-cell RNA sequencing, we profiled ~276,000 peripheral blood mononuclear cells from 33 children with SLE with different degrees of disease activity and 11 matched controls. Increased expression of interferon-stimulated genes (ISGs) distinguished cells from children with SLE from healthy control cells. The high ISG expression signature (ISG
Recommended Citation
Nehar-Belaid D,
Hong S,
Marches R,
Chen G,
Bolisetty M,
Baisch J,
Walters L,
Punaro M,
Rossi R,
Chung C,
Huynh R,
Singh P,
Flynn W,
Tabanor-Gayle J,
Kuchipudi N,
Mejias A,
Collet M,
Lucido A,
Palucka K,
Robson P,
Lakshminarayanan S,
Ramilo O,
Wright T,
Pascual V,
Banchereau J.
Mapping systemic lupus erythematosus heterogeneity at the single-cell level. Nat Immunol 2020 Sep; 21(9):1094-1106
Comments
We gratefully acknowledge the contribution of Flow Cytometry, Single Cell Biology and Genome Technologies Scientific Services at The Jackson Laboratory. In addition, we thank JAX Research IT (particularly Sandeep Namburi) for the support with maintaining the R Shiny application.