Document Type
Article
Publication Date
1-21-2020
Keywords
JMG
JAX Source
Sci Rep 2020 Jan 21; 10(1):828
Volume
10
Issue
1
First Page
828
Last Page
828
ISSN
2045-2322
PMID
31964978
DOI
https://doi.org/10.1038/s41598-020-57663-x
Abstract
The aging brain is associated with significant changes in physiology that alter the tissue microenvironment of the central nervous system (CNS). In the aged CNS, increased demyelination has been associated with astrocyte hypertrophy and aging has been implicated as a basis for these pathological changes. Aging tissues accumulate chronic cellular stress, which can lead to the development of a pro-inflammatory phenotype that can be associated with cellular senescence. Herein, we provide evidence that astrocytes aged in culture develop a spontaneous pro-inflammatory and senescence-like phenotype. We found that extracellular vesicles (EVs) from young astrocyte were sufficient to convey support for oligodendrocyte differentiation while this support was lost by EVs from aged astrocytes. Importantly, the negative influence of culture age on astrocytes, and their cognate EVs, could be countered by treatment with rapamycin. Comparative proteomic analysis of EVs from young and aged astrocytes revealed peptide repertoires unique to each age. Taken together, these findings provide new information on the contribution of EVs as potent mediators by which astrocytes can extert changing influence in either the disease or aged brain.
Recommended Citation
Willis C,
Nicaise A,
Bongarzone E,
Givogri M,
Reiter C,
Heintz O,
Jellison E,
Sutter P,
TeHennepe G,
Ananda G,
Vella A,
Crocker S.
Astrocyte Support for Oligodendrocyte Differentiation can be Conveyed via Extracellular Vesicles but Diminishes with Age. Sci Rep 2020 Jan 21; 10(1):828
Comments
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