Document Type

Article

Publication Date

11-2020

Keywords

JMG, JAXCC

JAX Source

Aging Cell 2020 Nov; 19(11):e13269

First Page

13269

Last Page

13269

ISSN

1474-9726

PMID

33145977

DOI

https://doi.org/10.1111/acel.13269

Grant

AG022308; CA034196

Abstract

To see if variations in timing of rapamycin (Rapa), administered to middle aged mice starting at 20 months, would lead to different survival outcomes, we compared three dosing regimens. Initiation of Rapa at 42 ppm increased survival significantly in both male and female mice. Exposure to Rapa for a 3-month period led to significant longevity benefit in males only. Protocols in which each month of Rapa treatment was followed by a month without Rapa exposure were also effective in both sexes, though this approach was less effective than continuous exposure in female mice. Interpretation of these results is made more complicated by unanticipated variation in patterns of weight gain, prior to the initiation of the Rapa treatment, presumably due to the use of drug-free food from two different suppliers. The experimental design included tests of four other drugs, minocycline, β-guanidinopropionic acid, MitoQ, and 17-dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG), but none of these led to a change in survival in either sex.

Comments

This is an open access article under the terms of the Creative Commons Attribution License.

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