Document Type
Article
Publication Date
10-20-2020
Publication Title
Cell Rep
Keywords
JGM
JAX Source
Cell Rep 2020 Oct 20; 33:108293
Volume
33
Issue
3
First Page
108293
Last Page
108293
ISSN
2211-1247
PMID
33086062
Abstract
Histone methyltransferase KMT2D harbors frequent loss-of-function somatic point mutations in several tumor types, including melanoma. Here, we identify KMT2D as a potent tumor suppressor in melanoma through an in vivo epigenome-focused pooled RNAi screen and confirm the finding by using a genetically engineered mouse model (GEMM) based on conditional and melanocyte-specific deletion of KMT2D. KMT2D-deficient tumors show substantial reprogramming of key metabolic pathways, including glycolysis. KMT2D deficiency aberrantly upregulates glycolysis enzymes, intermediate metabolites, and glucose consumption rates. Mechanistically, KMT2D loss causes genome-wide reduction of H3K4me1-marked active enhancer chromatin states. Enhancer loss and subsequent repression of IGFBP5 activates IGF1R-AKT to increase glycolysis in KMT2D-deficient cells. Pharmacological inhibition of glycolysis and insulin growth factor (IGF) signaling reduce proliferation and tumorigenesis preferentially in KMT2D-deficient cells. We conclude that KMT2D loss promotes tumorigenesis by facilitating an increased use of the glycolysis pathway for enhanced biomass needs via enhancer reprogramming, thus presenting an opportunity for therapeutic intervention through glycolysis or IGF pathway inhibitors.
Recommended Citation
Maitituoheti M,
Keung E,
Tang M,
Yan L,
Alam H,
Han G,
Singh A,
Raman A,
Terranova C,
Sarkar S,
Orouji E,
Amin S,
Sharma S,
Williams M,
Samant N,
Dhamdhere M,
Zheng N,
Shah T,
Shah A,
Axelrad J,
Anvar N,
Lin Y,
Jiang S,
Chang E,
Ingram D,
Wang W,
Lazar A,
Lee M,
Muller F,
Wang L,
Ying H,
Rai K.
Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma. Cell Rep 2020 Oct 20; 33:108293
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