Document Type

Article

Publication Date

3-1-2020

Keywords

JMG, JGM, JAXCC

JAX Source

Genetics 2020 Mar; 214(3):719-733

Volume

214

Issue

3

First Page

719

Last Page

733

ISSN

1943-2631

PMID

31896565

DOI

https://doi.org/10.1534/genetics.119.303013

Grant

DA043809

Abstract

The microbiome influences health and disease through complex networks of host genetics, genomics, microbes, and environment. Identifying the mechanisms of these interactions has remained challenging. Systems genetics in laboratory mice (Mus musculus) enables data-driven discovery of biological network components and mechanisms of host-microbial interactions underlying disease phenotypes. To examine the interplay among the whole host genome, transcriptome, and microbiome, we mapped QTL and correlated the abundance of cecal messenger RNA, luminal microflora, physiology, and behavior in a highly diverse Collaborative Cross breeding population. One such relationship, regulated by a variant on chromosome 7, was the association of Odoribacter (Bacteroidales) abundance and sleep phenotypes. In a test of this association in the BKS.Cg-Dock7m +/+ Leprdb/J mouse model of obesity and diabetes, known to have abnormal sleep and colonization by Odoribacter, treatment with antibiotics altered sleep in a genotype-dependent fashion. The many other relationships extracted from this study can be used to interrogate other diseases, microbes, and mechanisms.

Comments

Jennifer Ryan, Neil Cole, Christine Rosales, Laura Anderson and Samantha 636 Burrill conducted db sleep studies and dissections.

This open access article is licensed under a Creative Commons Attribution 4.0 International License

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