Techniques for the generation of humanized mouse models for immuno-oncology.
Document Type
Article
Publication Date
2020
Keywords
JGM
JAX Source
Meth Enzymol 2020; 636:351-368
Volume
636
First Page
351
Last Page
368
ISSN
1557-7988
PMID
32178826
DOI
https://doi.org/10.1016/bs.mie.2019.06.003
Grant
CA195712,CA219880,Department of Defense (W81XWH-17-1-0010) and The Jackson Laboratory
Abstract
Mouse models of human cancer have been used extensively to circumvent the complexity in human patients. However, murine models often inadequately recapitulate the human cancer-immune interface partly due to important differences between mouse and human immune systems. Immunodeficient mice when transplanted with CD34+ hematopoietic progenitor cells (HPCs) develop multilineage human immune cells. While there remain limitations, efforts have been made to improve the function of human immune system. Thus, humanized mice, defined as mice with human immune system, have become an emerging model to study human cancers. Humanized mouse models have been used for various areas of cancer research including adoptive transfer of chimeric antigen receptor (CAR)-modified T cells, neoantigen vaccination to increase T cell repertoire and reprograming tumor microenvironment. Here, we describe the essential techniques to generate humanized mouse models for immuno-oncology studies.
Recommended Citation
Yu CI,
Marches F,
Wu T,
Martinek J,
Palucka K.
Techniques for the generation of humanized mouse models for immuno-oncology. Meth Enzymol 2020; 636:351-368
Comments
We thank patients and healthy donors for participation in our studies over the years. We thank Dr. Jim Keck and Dr. Lenny Shultz for collaboration and Vanessa K. P. Oliveira for help with experiments. Support over the years came from Baylor Institute for Immunology Research and Baylor University Medical Center Foundation.