Document Type
Article
Publication Date
5-1-2020
Keywords
JGM
JAX Source
Nat Commun 2020 May 1; 11(1):2120
Volume
11
Issue
1
First Page
2120
Last Page
2120
ISSN
2041-1723
PMID
32358536
DOI
https://doi.org/10.1038/s41467-020-15987-2
Abstract
The human genome is extensively folded into 3-dimensional organization. However, the detailed 3D chromatin folding structures have not been fully visualized due to the lack of robust and ultra-resolution imaging capability. Here, we report the development of an electron microscopy method that combines serial block-face scanning electron microscopy with in situ hybridization (3D-EMISH) to visualize 3D chromatin folding at targeted genomic regions with ultra-resolution (5 × 5 × 30 nm in xyz dimensions) that is superior to the current super-resolution by fluorescence light microscopy. We apply 3D-EMISH to human lymphoblastoid cells at a 1.7 Mb segment of the genome and visualize a large number of distinctive 3D chromatin folding structures in ultra-resolution. We further quantitatively characterize the reconstituted chromatin folding structures by identifying sub-domains, and uncover a high level heterogeneity of chromatin folding ultrastructures in individual nuclei, suggestive of extensive dynamic fluidity in 3D chromatin states.
Recommended Citation
Trzaskoma P,
Ruszczycki B,
Lee B,
Pels K,
Krawczyk K,
Bokota G,
Szczepankiewicz A,
Aaron J,
Walczak A,
Śliwińska M,
Magalska A,
Kadlof M,
Wolny A,
Parteka Z,
Arabasz S,
Kiss-Arabasz M,
Plewczyński D,
Ruan Y,
Wilczyński G.
Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization. Nat Commun 2020 May 1; 11(1):2120
Comments
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