JGM, Activin Receptors, Type II, Animals, Biopsy, Bone Morphogenetic Proteins, Disease Models, Animal, Embryo Implantation, Endometrium, Estrogens, Female, Humans, Infertility, Female, Mice, Mice, Knockout, Pregnancy, Signal Transduction, Smad1 Protein, Smad5 Protein
Nat Commun 2021 Jun 7; 12(1):3386
During early pregnancy in the mouse, nidatory estrogen (E2) stimulates endometrial receptivity by activating a network of signaling pathways that is not yet fully characterized. Here, we report that bone morphogenetic proteins (BMPs) control endometrial receptivity via a conserved activin receptor type 2 A (ACVR2A) and SMAD1/5 signaling pathway. Mice were generated to contain single or double conditional deletion of SMAD1/5 and ACVR2A/ACVR2B receptors using progesterone receptor (PR)-cre. Female mice with SMAD1/5 deletion display endometrial defects that result in the development of cystic endometrial glands, a hyperproliferative endometrial epithelium during the window of implantation, and impaired apicobasal transformation that prevents embryo implantation and leads to infertility. Analysis of Acvr2a-PRcre and Acvr2b-PRcre pregnant mice determined that BMP signaling occurs via ACVR2A and that ACVR2B is dispensable during embryo implantation. Therefore, BMPs signal through a conserved endometrial ACVR2A/SMAD1/5 pathway that promotes endometrial receptivity during embryo implantation.
Monsivais, Diana; Nagashima, Takashi; Prunskaite-Hyyryläinen, Renata; Nozawa, Kaori; Shimada, Keisuke; Tang, Suni; Hamor, Clark; Agno, Julio E; Chen, Fengju; Masand, Ramya P; Young, Steven L; Creighton, Chad J; DeMayo, Francesco J; Ikawa, Masahito; Lee, Se-Jin; and Matzuk, Martin M, "Endometrial receptivity and implantation require uterine BMP signaling through an ACVR2A-SMAD1/SMAD5 axis." (2021). Faculty Research 2021. 130.