STAR Protoc 2021 Dec 17; 2:100866
This protocol features parallel isolation of myocytes and non-myocytes from murine hearts. It was designed with considerations for (1) time required to extract cardiac cells, (2) cell viability, and (3) protocol scalability. Here, a peristaltic pump and 3D-printed elements are combined to perfuse the heart with enzymes to dissociate cells. Myocytes and non-myocytes extracted using this protocol are separated by centrifugation and/or fluorescence-activated cell sorting for use in downstream applications including single-cell omics or other bio-molecular analyses. For complete details on the use and execution of this protocol, please refer to McLellan et al. (2020).
Farrugia, Gabriella E; McLellan, Michael A; Weeks, Kate L; Matsumoto, Aya; Cohen, Charles D; Krstevski, Crisdion; Gaynor, Taylah L; Parslow, Adam C; McMullen, Julie R; and Pinto, Alexander R, "A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts." (2021). Faculty Research 2021. 227.