Title
A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts.
Document Type
Article
Publication Date
12-17-2021
Publication Title
STAR Protoc
JAX Source
STAR Protoc 2021 Dec 17; 2:100866
Volume
2
Issue
4
First Page
100866
Last Page
100866
ISSN
2666-1667
PMID
34647038
DOI
https://doi.org/10.1016/j.xpro.2021.100866
Abstract
This protocol features parallel isolation of myocytes and non-myocytes from murine hearts. It was designed with considerations for (1) time required to extract cardiac cells, (2) cell viability, and (3) protocol scalability. Here, a peristaltic pump and 3D-printed elements are combined to perfuse the heart with enzymes to dissociate cells. Myocytes and non-myocytes extracted using this protocol are separated by centrifugation and/or fluorescence-activated cell sorting for use in downstream applications including single-cell omics or other bio-molecular analyses. For complete details on the use and execution of this protocol, please refer to McLellan et al. (2020).
Recommended Citation
Farrugia, Gabriella E; McLellan, Michael A; Weeks, Kate L; Matsumoto, Aya; Cohen, Charles D; Krstevski, Crisdion; Gaynor, Taylah L; Parslow, Adam C; McMullen, Julie R; and Pinto, Alexander R, "A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts." (2021). Faculty Research 2021. 227.
https://mouseion.jax.org/stfb2021/227
Comments
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