Document Type

Article

Publication Date

1-11-2022

Publication Title

Cell Rep

Keywords

JMG, Animals, Autism Spectrum Disorder, Deep Learning, Exploratory Behavior, Gait, Genome-Wide Association Study, Mice, Movement, Nerve Net, Open Field Test, Postural Balance

JAX Source

Cell Rep 2022 Jan 11; 38(2):110231

Volume

38

Issue

2

First Page

110231

Last Page

110231

ISSN

2211-1247

PMID

35021077

DOI

https://doi.org/10.1016/j.celrep.2021.110231

Grant

Jackson Laboratory Directors Innovation Fund, DA041668, DA048634

Abstract

Gait and posture are often perturbed in many neurological, neuromuscular, and neuropsychiatric conditions. Rodents provide a tractable model for elucidating disease mechanisms and interventions. Here, we develop a neural-network-based assay that adopts the commonly used open field apparatus for mouse gait and posture analysis. We quantitate both with high precision across 62 strains of mice. We characterize four mutants with known gait deficits and demonstrate that multiple autism spectrum disorder (ASD) models show gait and posture deficits, implying this is a general feature of ASD. Mouse gait and posture measures are highly heritable and fall into three distinct classes. We conduct a genome-wide association study to define the genetic architecture of stride-level mouse movement in the open field. We provide a method for gait and posture extraction from the open field and one of the largest laboratory mouse gait and posture data resources for the research community.

Comments

We thank members of the Kumar lab for helpful advice and Taneli Helenius for editing.Wethank Massimo Daul for the preliminary work on corner detection of our open fields. We thank Dr. Vivek Philip for advice on GWAS analysis. We thank JAX Information Technology team members Edwardo Zaborowski, Shane Sanders, Rich Brey, David McKenzie, and Jason Macklin for infrastructure support.

This is an open access article under the CC BY-NC-ND license.

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