Biofabrication of 3D breast cancer models for dissecting the cytotoxic response of human T cells expressing engineered MAIT cell receptors.

Document Type

Article

Publication Date

9-29-2022

Publication Title

Biofabrication

Keywords

JGM, Breast Neoplasms, Cytokines, Female, Fibrin, Granzymes, Humans, Interferon-gamma, Interleukin-13, Ligands, Minor Histocompatibility Antigens, Mucosal-Associated Invariant T Cells, Receptors, Antigen, T-Cell, T-Lymphocytes, Tumor Microenvironment

JAX Source

Biofabrication. 2022;14(4):044105.

Volume

14

Issue

4

ISSN

1758-5090

PMID

36108605

DOI

https://doi.org/10.1088/1758-5090/ac925a

Grant

This work was supported by NSF Awards 1914885 (I T O) and 1624515 (I T O), 2236 CoCir- culation2 of TUBITAK Award 121C359 (I T O), and NIH Award R21 CA224422 01A1 (I T O and D U).

Abstract

Immunotherapy has revolutionized cancer treatment with the advent of advanced cell engineering techniques aimed at targeted therapy with reduced systemic toxicity. However, understanding the underlying immune-cancer interactions require development of advanced three-dimensional (3D) models of human tissues. In this study, we fabricated 3D tumor models with increasing complexity to study the cytotoxic responses of CD8

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