Document Type

Article

Publication Date

10-27-2022

Publication Title

NPJ Genom Med

Keywords

JGM

JAX Source

NPJ Genom Med. 2022;7(1):63

Volume

7

Issue

1

First Page

63

ISSN

2056-7944

PMID

36302783

DOI

https://doi.org/10.1038/s41525-022-00333-w

Grant

The authors would like to acknowledge grant support from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (grant number: 2018R1D1A1B07045601 to G.J.); the National Research Foundation of Korea (NRF) grants funded by the Korean government (MSIT) (grant numbers: 2021R1A2C3008021 and 2021R1A4A1029097 to S.-Y.C.); and the International Research & Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT (grant number: 2015K1A4A3047851 to J.K.). C.L. was a distinguished Ewha Womans University Professor supported in part by the Ewha Womans University Research grant (2017–2020). The authors thank Kyue-Yim Lee for NTA measurement education, Ms. Jane Cha for the illustration and Dr. Stephen Sampson for careful editing of this manuscript.

Abstract

Pancreatic cancer exhibits a characteristic tumor microenvironment (TME) due to enhanced fibrosis and hypoxia and is particularly resistant to conventional chemotherapy. However, the molecular mechanisms underlying TME-associated treatment resistance in pancreatic cancer are not fully understood. Here, we developed an in vitro TME mimic system comprising pancreatic cancer cells, fibroblasts and immune cells, and a stress condition, including hypoxia and gemcitabine. Cells with high viability under stress showed evidence of increased direct cell-to-cell transfer of biomolecules. The resulting derivative cells (CD44

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