Document Type
Article
Publication Date
12-1-2022
Publication Title
Cell Stem Cell
Keywords
JGM, SS1, Humans, Induced Pluripotent Stem Cells, Cell Differentiation, Gene Editing, Biological Assay
JAX Source
Cell Stem Cell. 2022;29(12):1685-702.e22.
Volume
29
Issue
12
First Page
1685
Last Page
1702
ISSN
1875-9777
PMID
36459969
DOI
https://doi.org/10.1016/j.stem.2022.11.004
Abstract
Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. These studies identified KOLF2.1J as an all-around well-performing iPSC line. We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head comparisons with their own preferred iPSC lines across a diverse range of differentiation protocols and functional assays. On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field.
Recommended Citation
Pantazis C,
Yang A,
Lara E,
McDonough J,
Blauwendraat C,
Peng L,
Oguro H,
Kanaujiya J,
Zou J,
Sebesta D,
Pratt G,
Cross E,
Blockwick J,
Buxton P,
Kinner-Bibeau L,
Medura C,
Tompkins C,
Hughes S,
Santiana M,
Faghri F,
Nalls M,
Vitale D,
Ballard S,
Qi Y,
Ramos D,
Anderson K,
Stadler J,
Narayan P,
Papademetriou J,
Reilly L,
Nelson M,
Aggarwal S,
Rosen L,
Kirwan P,
Pisupati V,
Coon S,
Scholz S,
Priebe T,
Öttl M,
Dong J,
Meijer M,
Janssen L,
Lourenco V,
van der Kant R,
Crusius D,
Paquet D,
Raulin A,
Bu G,
Held A,
Wainger B,
Gabriele R,
Casey J,
Wray S,
Abu-Bonsrah D,
Parish C,
Beccari M,
Cleveland D,
Li E,
Rose I,
Kampmann M,
Calatayud Aristoy C,
Verstreken P,
Heinrich L,
Chen M,
Schüle B,
Dou D,
Holzbaur E,
Zanellati M,
Basundra R,
Deshmukh M,
Cohen S,
Khanna R,
Raman M,
Nevin Z,
Matia M,
Van Lent J,
Timmerman V,
Conklin B,
Johnson Chase K,
Zhang K,
Funes S,
Bosco D,
Erlebach L,
Welzer M,
Kronenberg-Versteeg D,
Lyu G,
Arenas E,
Coccia E,
Sarrafha L,
Ahfeldt T,
Marioni J,
Skarnes W,
Cookson M,
Ward M,
Merkle F.
A reference human induced pluripotent stem cell line for large-scale collaborative studies. Cell Stem Cell. 2022;29(12):1685-702.e22.
Comments
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).