Document Type

Article

Publication Date

5-25-2022

Publication Title

Sci Data

Keywords

JMG, Animals, Brain, Databases, Factual, Mice, X-Ray Microtomography

JAX Source

Sci Data 2022 May 25; 9(1):230

Volume

9

Issue

1

First Page

230

Last Page

230

ISSN

2052-4463

PMID

35614082

DOI

https://doi.org/10.1038/s41597-022-01338-x

Abstract

Complex morphological traits are the product of many genes with transient or lasting developmental effects that interact in anatomical context. Mouse models are a key resource for disentangling such effects, because they offer myriad tools for manipulating the genome in a controlled environment. Unfortunately, phenotypic data are often obtained using laboratory-specific protocols, resulting in self-contained datasets that are difficult to relate to one another for larger scale analyses. To enable meta-analyses of morphological variation, particularly in the craniofacial complex and brain, we created MusMorph, a database of standardized mouse morphology data spanning numerous genotypes and developmental stages, including E10.5, E11.5, E14.5, E15.5, E18.5, and adulthood. To standardize data collection, we implemented an atlas-based phenotyping pipeline that combines techniques from image registration, deep learning, and morphometrics. Alongside stage-specific atlases, we provide aligned micro-computed tomography images, dense anatomical landmarks, and segmentations (if available) for each specimen (N = 10,056). Our workflow is open-source to encourage transparency and reproducible data collection. The MusMorph data and scripts are available on FaceBase ( www.facebase.org , https://doi.org/10.25550/3-HXMC ) and GitHub ( https://github.com/jaydevine/MusMorph ).

Comments

This article is licensed under a Creative Commons Attribution 4.0 International License.

Share

COinS