Dendritic Cell Differentiation from Human Induced Pluripotent Stem Cells: Challenges and Progress.

Document Type

Article

Publication Date

5-1-2022

Publication Title

Stem cells and development

Keywords

JGM, Bone Marrow, Cell Differentiation, Dendritic Cells, Humans, Induced Pluripotent Stem Cells

JAX Source

Stem Cells Dev 2022 May; 31(9-10):207-220

Volume

31

Issue

9-10

First Page

207

Last Page

220

ISSN

1557-8534

PMID

35316109

DOI

https://doi.org/10.1089/scd.2021.0305

Abstract

Dendritic cells (DCs) are the major antigen-presenting cells of the immune system responsible for initiating and coordinating immune responses. These abilities provide potential for several clinical applications, such as the development of immunogenic vaccines. However, difficulty in obtaining DCs from conventional sources, such as bone marrow, peripheral blood, and cord blood, significantly hinders routine application. The use of human induced pluripotent stem cells (hiPSCs) is a valuable alternative for generating sufficient numbers of DCs to be used in basic and preclinical studies. Despite the many challenges that must be overcome to achieve an efficient protocol for obtaining the major DC types from hiPSCs, recent progress has been made. In this study, we review the current state of developing DCs from hiPSCs, as well as the key elements required to enable the routine use of hiPSC-derived DCs in preclinical and clinical assays.

Comments

We acknowledge Mr. Richie Huynh, Mr. Stephen Sampson, and Prof. Dr. Yusuf Cxetin Kocaefe for reading the article and also for their valuable suggestions.

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