Document Type

Article

Publication Date

6-1-2022

Publication Title

Pain

Keywords

JMG, Animals, Freund's Adjuvant, Mice, Mice, Knockout, Nociception, Pain, Pain Measurement

JAX Source

Pain 2022 Jun 1; 163(6):1139-1157

Volume

163

Issue

6

First Page

1139

Last Page

1157

ISSN

1872-6623

PMID

35552317

DOI

https://doi.org/10.1097/j.pain.0000000000002481

Grant

OD023222

Abstract

ABSTRACT: Identifying the genetic determinants of pain is a scientific imperative given the magnitude of the global health burden that pain causes. Here, we report a genetic screen for nociception, performed under the auspices of the International Mouse Phenotyping Consortium. A biased set of 110 single-gene knockout mouse strains was screened for 1 or more nociception and hypersensitivity assays, including chemical nociception (formalin) and mechanical and thermal nociception (von Frey filaments and Hargreaves tests, respectively), with or without an inflammatory agent (complete Freund's adjuvant). We identified 13 single-gene knockout strains with altered nocifensive behavior in 1 or more assays. All these novel mouse models are openly available to the scientific community to study gene function. Two of the 13 genes (Gria1 and Htr3a) have been previously reported with nociception-related phenotypes in genetically engineered mouse strains and represent useful benchmarking standards. One of the 13 genes (Cnrip1) is known from human studies to play a role in pain modulation and the knockout mouse reported herein can be used to explore this function further. The remaining 10 genes (Abhd13, Alg6, BC048562, Cgnl1, Cp, Mmp16, Oxa1l, Tecpr2, Trim14, and Trim2) reveal novel pathways involved in nociception and may provide new knowledge to better understand genetic mechanisms of inflammatory pain and to serve as models for therapeutic target validation and drug development.

Comments

The authors thank Cynthia Smith for her assistance with MouseMine searches on the Mouse Genome Database and Martin Ringwald for his assistance with Gene Expression Database (GXD) searches. The authors thank Federico Lopez Gomez for his assistance developing Solr queries for the IMPC development site. The authors are very grateful to Judith Morgan and Debbie Kelley for their assistance in assembling and editing the manuscript.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0.

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