Document Type
Article
Publication Date
8-1-2025
Original Citation
Reagan A,
MacLean M,
Cossette T,
Howell G.
Retinal vascular dysfunction in the Mthfr(677C>T) mouse model of cerebrovascular disease Alzheimers Dement. 2025;21(8):e70501.
Keywords
JMG, SS1, Animals, Methylenetetrahydrofolate Reductase (NADPH2), Disease Models, Animal, Cerebrovascular Disorders, Mice, Retinal Vessels, Male, Female, Brain, Retina, Mice, Transgenic, Electroretinography, Mice, Inbred C57BL
JAX Source
Alzheimers Dement. 2025;21(8):e70501.
ISSN
1552-5279
PMID
40741711
DOI
https://doi.org/10.1002/alz.70501
Abstract
INTRODUCTION: Investigations of retinal biomarkers for Alzheimer's disease (AD) and AD and related dementias (ADRD), has increased significantly. We examine retinal vascular health in a mouse containing the ADRD risk variant Mthfr
METHODS: Morphology and function of retinal vasculature and neurons were assessed using in vivo imaging, immunohistochemistry, and pattern electroretinography. RNAscope and proteomics were employed to determine Mthfr gene expression and differential protein expression in mice carrying Mthfr
RESULTS: Mice show age- and sex-dependent retinal vascular deficits, displaying similarities to previously published brain data. Mthfr is widely expressed and co-localizes with vascular cell markers. Proteomics identified common molecular signatures across the brain and retina.
DISCUSSION: Results demonstrate that Mthfr-dependent vascular phenotypes occur in brain and retina similarly. These data suggest that assessing age and genetic-driven changes within retinal vasculature represents a minimally invasive method to predict AD-related cerebrovascular damage.
HIGHLIGHTS: Mthfr
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