Comparative genomics to determine the genotype responsible for a bacillus subtilis mutant phenotype.

Amber Acquaye

Document Type

Article

Publication Date

Summer 2016

JAX Location

In: Student Reports, Summer 2016, Jackson Laboratory

Abstract

Bacillus subtilis is often used as a model for understanding the bacterial cell cycle. This investigation serves to isolate and identify the mutation responsible for a B. subtilis train's resistance to cell cycle inhibition by a bacteriophage. By using comparative genomics to compare the parent and daughter B. subtilis genomes, single nucleotide polymorphisms (SNPs) were located and characterized. The illumina whole genome shotgun sequence of two B. subtilis strins, a parent and mutant strain, were each mapped to a published completed . subtilis genome. SNPs were characterized (gene coding or non-coding regions, aminoacid change or same amino acid encoded) and used to identify the gene responsible for resistance to phage inhibition. A SNP in the FtsA gene, which encodes a cell division protein responsible for holding the Z ring (proteins that dentate the mid-cell) in place along the membrane, was identified to be the gene housing the mutation causing phage resistance.

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