Role of PRDM9 KRAB domain in H3K4 trimethylation at hotspot loci.

Naomi Bronkema

Document Type

Article

Publication Date

Summer 2017

JAX Location

In: Student Reports, Summer 2017, Jackson Laboratory

Abstract

Meiosis and genetic recombination are fundamental for sexual reproduction and increasing genetic diversity within a population. PRDM9 is a crucial protein for the successful completion of meiosis and genetic recombination. While PRDM9 is known to have a major role in defining the location of recombination hotspots through the placement of H3K4me3 localized where a DSB will occur, much less is known about the exact mechanisms. The purpose of this experiment was to determine which protein domains of PRDM9 are necessary for H3K4me3 at hotspots. We used a cell-based system to transfect wild-type and mutated alleles of PRDM9 to determine the change in H3K4me3 levels. Contrary to previously published results, our results suggest that the conserved KRAB domain is not necessary for H3K4me3. Further experiments will test where the KRAB domain is necessary for the completion of genetic recombination.

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