Is RNA editing involved in the full recovery of kidneys in Black Bears after hibernation.

Document Type

Article

Publication Date

Summer 2017

JAX Location

In: Student Reports, Summer 2017, Jackson Laboratory

Abstract

My research focused on determining whether RNA editing was responsible for the differences in base pairs between the Black Bear genome sequence and the RNA sequence in the kidney. Thirty-five cases of RNA editing were observed in Dr. Ron Korstanje's lab by analyzing and comparing the RNA and genome sequences for a change of base pairs alone. In this study, I utilized Integrative Genomics Viewer, Polymerase Chain reactions, Electrophoresis Gels, and Sanger Sequencing on three Fall and three Spring black bear samples to confirm eight cases of RNA editing. I did so in an attempt to fmd a correlation between the process of RNA editing and the Black Bear's ability to reverse kidney damage after hibernation.

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