The Effects of AlCF Knock Out on Stressed Mouse Kidneys

Bailey Hixon

Document Type

Article

Publication Date

Summer 2018

JAX Location

In: Student Reports, Summer 2018, The Jackson Laboratory

Abstract

A1CF, encoding the RNA-binding protein Apobec-1 complementation factor, has been found to be associated with kidney function in human genome-wide association studies. Previous studies in our lab showed that A 1 CF is located in the proximal tubule of the kidney, but the role of this protein is unknown. Rip-Seq analysis suggests that 2 A 1 CF is associated with mitochondrial function and ER stress. Proximal tubule epithelial cells are rich with mitochondria to provide energy for solute transport across a strong electrochemical gradient. Mitochondrial dysfunction has been connected with an increase in fibrosis and apoptosis, phenotypes present in A 1 cf knock down models. Therefore, the objective of this study was to investigate the effects of A 1cf knock down on mitochondrial function in cell culture, followed by determining if A 1 CF affected mitochondrial function is associated with apoptosis, fibrosis, and ER stress in a folic acid, stressed induced, mouse kidney.

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