The role of Oit3 in embryonic development

Lenka Slamova

Document Type

Article

Publication Date

Summer 2022

Keywords

JMG

JAX Location

In: Student Reports, Summer 2022, The Jackson Laboratory

Abstract

In an analysis of breeding for Oit3 knockouts in the in the 129S1/SvImJ genetic background at our lab, we found that mating of heterozygous animals results in 12.7% of homozygous knockout animals instead of the expected 25% suggesting that Oit3 may be involved in embryonic development. To investigate the role of Oit3 in development, we set up timed mating of heterozygous animals, collected E18.5 embryos, genotyped embryos at this time point and phenotyped the mutants. Due to issues with genotyping, we were unable to evaluate the ratio of genotypes at E18.5. In micro-CT images of three mutants and three wild-type controls that we analyzed so far, we may have found an abnormal heart phenotype which will be investigated further by organ isolation in 3D to look at volumetric and structural differences and imaging of at least four more mutant embryos. We found no other abnormal morphological phenotypes in the mutants.

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