Cell Cycle Changes Following TPHP Exposure of Induced Pluripotent Stem Cells

Lacie Low

Document Type

Article

Publication Date

Summer 2023

Keywords

JMG

JAX Location

In: Student Reports, Summer 2023, The Jackson Laboratory

Abstract

Pluripotent stem cells have the unique ability to differentiate into the three primary germ layers, allowing them to give rise to all future cell lineages and subsequent embryonic development. There are early data to suggest that environmental toxicants, such as triphenyl phosphate (TPHP), interfere with this differentiation and division process potentially giving rise to dangerous health effects. This project examined the impact of TPHP exposure on undifferentiated mouse induced pluripotent stem cells (iPSCs). Based on published data and our own preliminary observations, we hypothesized that TPHP exposure would interfere with iPSC cell cycle. Our results provide data to support the presence of TPHP induced underlying adverse effects of cell cycle interference in pluripotent stem cells. This is in tandem with how these effects differ due to innate genetic diversity across experimental strains, potentially providing useful references in the interest of evaluating chemical safety for developing tissues. Experimental results show a general trend in the reduction of the population of iPSCs in G2 of the cell cycle along with the increase of populations in G1. The variable responses amongst different iPSC strains support the argument for the requirement of genetic variability in toxicology studies.

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