Mapping genetic effects of diverse mESC lines on cellular phenotypes with Census-seq
Document Type
Article
Publication Date
8-9-2024
Keywords
JMG
JAX Location
In: Student Reports, Summer 2024, The Jackson Laboratory
Sponsor
Selcan Aydin, Hannah Dewey, and Steven Munger, Ph.D.
Abstract
Population-scale phenotyping of arrayed cell lines is currently expensive and complex; one solution is to combine cells from many individuals into a "cell village." And while a number of groups have applied this approach to human cell populations, and have developed computational methods including Census-seq to estimate the relative proportion of cells from each individual, these approaches have not yet been validated in mouse cell models, despite their potential to increase genetic mapping power and enable validation of risk genes associated with complex disease. To implement Census-seq for mouse models, first a pipeline must be built to make it accessible to mESC data, then it needs to be thoroughly tested: focusing on Census-seq's accuracy with varying sample sizes, sequencing depth, and genetic variation.
Recommended Citation
Rea, Madeline, "Mapping genetic effects of diverse mESC lines on cellular phenotypes with Census-seq" (2024). Summer and Academic Year Student Reports. 2775.
https://mouseion.jax.org/strp/2775