Characterizing Lung Cancer Metastasis in the Novel KPDT Mouse Model

Travis Beckett

Document Type

Article

Publication Date

8-9-2024

Keywords

JMG

JAX Location

In: Student Reports, Summer 2024, The Jackson Laboratory

Abstract

Non-small cell lung cancer, lung adenocarcinoma (LUAD) in particular, is the most prevalent form of lung cancer and possesses the highest rate of lethality of all oncogenic diseases. Metastatic progression is a defining feature of later stage cancer progression and is the primary cause for cancer deaths. Despite the overwhelming presence and lethality of LUAD in humans, established mouse models do not reliably develop metastatic disease. This project aimed to characterize metastatic potential in the novel KPDT mouse model through histological and flow cytometric methods. Additionally, a transcriptomic analysis was conducted from bulk RNA sequencing data to identify differentially expressed genes in two KPDT models with or without Dicer1 inhibition. The results confirmed the reliability of the mT/mG double fluorescent Cre reporter to differentiate healthy and cancerous tissue in tumor-bearing mice. Histological and flow cytometry experiments produced refined laboratory procedures to be used in yielding reproducible results for future analyses. The Transcriptomic analysis revealed many significantly differentiated phenotypes capable of being the subjects of therapeutics.

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