Cholesterol, 7-ketocholesterol and 25-hydroxycholesterol uptake studies and effect on 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity in human fibroblasts.

Authors

J L. Breslow
D A. Lothrop
D R. Spaulding
A A. Kandutsch

Document Type

Article

Publication Date

1975

Keywords

Biological-Transport, Cells-Cultured, Cholesterol: aa, me, Culture-Media, Fibroblasts: de, me, Human, Hydroxycholesterols: me, Hydroxymethylglutaryl-CoA-Reductases: ai, Infant-Newborn, Ketosteroids: me, Kinetics, Lipoproteins, Male, SUPPORT-U-S-GOVT-P-H-S, Thermodynamics, Time-Factors

First Page

10

Last Page

17

JAX Location

41,014

JAX Source

Biochim-Biophys-Acta. 1975 Jul 22; 398(1):10-7.

Abstract

In human fibroblasts two oxidized derivatives of cholesterol, 7-ketocholesterol and 25-hydroxycholesterol, but not cholesterol itself, are potent inhibitors of 3-hydroxy-3-methylglutaryl co-enzyme A reductase (mevalonate: NADP+ oxidoreductase (Co-enzyme A acylating), (EC 1.1.1.34), the rate-limiting enzyme in sterol biosynthesis. In addition, these derivatives of cholesterol are effective regulators in cells from homozygous familial hypercholesterolemic individuals. The differences in the inhibitory potencies of the sterols cannot be explained in terms of the amount of uptake into the cell.

Recommended Citation

Breslow JL, Lothrop DA, Spaulding DR, Kandutsch AA. Cholesterol, 7-ketocholesterol and 25-hydroxycholesterol uptake studies and effect on 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity in human fibroblasts. Biochim-Biophys-Acta. 1975 Jul 22; 398(1):10-7.