Culture of cells in beem capsules: a new technique for electron microscopic study of monolayer cultures.

Authors

J J. EppigFollow
E H. LeiterFollow
C Waymouth

Document Type

Article

Publication Date

1976

Keywords

Cell-Line, Cell-Membrane: ul, Cell-Nucleolus: ul, Cell-Nucleus: ul, Cells-Cultured: ul, Cytological-Technics, Cytoplasmic-Granules: ul, Insulin: se, Intercellular-Junctions: ul, Pancreas: cy, se, SUPPORT-U-S-GOVT-P-H-S

First Page

65

Last Page

73

JAX Location

41,156

JAX Source

In-Vitro. 1976 Jan; 12(1):65-73.

Abstract

A method is described for the monolayer cultivation of primary cell suspensions and established cell lines directly in carbon-coated BEEM capsules, BEEM capsules are routinely employed by electron microscopists in tissue embedding procedures; growing monolayer cultures directly on the lids of inverted BEEM capsules presents the obvious advantage of maintaining cell to cell and cell to substratum conthaets with a minimum of stress and damage in the preparative steps for electron microscopy.

Recommended Citation

Eppig JJ, Leiter EH, Waymouth C. Culture of cells in beem capsules: a new technique for electron microscopic study of monolayer cultures. In-Vitro. 1976 Jan; 12(1):65-73.