Autogenous regulation of RNA polymerase beta subunit synthesis in vitro.

Authors

R Fukuda
M Taketo
A Ishihama

Document Type

Article

Publication Date

1978

Keywords

Coliphages, DNA-Viral: me, Escherichia-Coli: en, Peptide-Elongation-Factors: bi, RNA-Polymerases: bi, Transduction-Genetic

First Page

4501

Last Page

4504

JAX Source

J-Biol-Chem. 1978 Jul 10; 253(13):4501-4.

Abstract

The effects of Escherichia coli RNA polymerase and its subassemblies and subunits on the in vitro synthesis of beta subunit directed by DNA from a lambda transducing phage lambdadrif+-6 were investigated. This phage carries the structural gene (rpoB) for beta subunit as well as the genes for EF (translation elongation factor)-Tu, some ribosomal proteins, and stable RNAs of the E. coli chromosome. Among the RNA polymerase proteins examined, the two oligomers, holoenzyme and alpha2beta complex, repressed the synthesis of only the beta subunit but not of other proteins encoded by the phage DNA. The results indicate that the expression of at least the betabeta' (rpoBC) operon is under autogenous regulation, in which both holoenzyme and alpha2beta complex function as regulatory molecules with repressor activity.

Recommended Citation

Fukuda R, Taketo M, Ishihama A. Autogenous regulation of RNA polymerase beta subunit synthesis in vitro. J-Biol-Chem. 1978 Jul 10; 253(13):4501-4.