Faculty Research 1970 - 1979
Effect of removal of the zona pellucida on subsequent development of mouse blastocysts in vitro.
Document Type
Article
Publication Date
1978
Keywords
Blastocyst: de, gd, Cells-Cultured, Female, Mice, Microscopy-Phase-Contrast, Ovum: ph, Plastics, Pronase, SUPPORT-U-S-GOVT-P-H-S, Zona-Pellucida: ph
First Page
137
Last Page
143
JAX Source
J Reprod Fertil 1978 Sep;54(1):137-43
Abstract
Removal of the zona pellucida allowed mouse blastocysts incapable of hatching in vitro to develop in culture. Blastocysts denuded by pronase always developed further than those of identical age that had hatched spontaneously. More blastocysts mechanically denuded and treated with pronase developed egg cylinder-like structures than did blastocysts mechanically denuded and not treated with pronase. Plastic support gave better development of blastocysts than did glass.
Recommended Citation
Konwinski M,
Solter D,
Koprowski H.
Effect of removal of the zona pellucida on subsequent development of mouse blastocysts in vitro. J Reprod Fertil 1978 Sep;54(1):137-43