The de-ubiquitinase UCH-L1 is an oncogene that drives the development of lymphoma in vivo by deregulating PHLPP1 and Akt signaling.
Document Type
Article
Publication Date
2010
Keywords
Cell-Line-Tumor, Humans, Lymphoma, Mice-Transgenic, Nuclear-Proteins, Oncogenes, Phosphoprotein-Phosphatases, Polymerase-Chain-Reaction, Proto-Oncogene-Proteins-c-akt, RNA-Interference, Signal-Transduction, Ubiquitin-Thiolesterase
First Page
1641
Last Page
1655
JAX Source
Leukemia 2010 Sep; 24(9):1641-55.
Abstract
De-ubiquitinating enzymes (DUBs) can reverse the modifications catalyzed by ubiquitin ligases and as such are believed to be important regulators of a variety of cellular processes. Several members of this protein family have been associated with human cancers; however, there is little evidence for a direct link between deregulated de-ubiquitination and neoplastic transformation. Ubiquitin C-terminal hydrolase (UCH)-L1 is a DUB of unknown function that is overexpressed in several human cancers, but whether it has oncogenic properties has not been established. To address this issue, we generated mice that overexpress UCH-L1 under the control of a ubiquitous promoter. Here, we show that UCH-L1 transgenic mice are prone to malignancy, primarily lymphomas and lung tumors. Furthermore, UCH-L1 overexpression strongly accelerated lymphomagenesis in Emu-myc transgenic mice. Aberrantly expressed UCH-L1 boosts signaling through the Akt pathway by downregulating the antagonistic phosphatase PHLPP1, an event that requires its de-ubiquitinase activity. These data provide the first in vivo evidence for DUB-driven oncogenesis and suggest that UCH-L1 hyperactivity deregulates normal Akt signaling.
Recommended Citation
Hussain S,
Foreman O,
Perkins SL,
Witzig TE,
Miles RR,
van DJ,
Galardy PJ.
The de-ubiquitinase UCH-L1 is an oncogene that drives the development of lymphoma in vivo by deregulating PHLPP1 and Akt signaling. Leukemia 2010 Sep; 24(9):1641-55.