Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity.

Document Type

Article

Publication Date

8-1-2010

Keywords

Animals, Bone Marrow Cells, Cell Cycle, Cell Differentiation, Cells, Cultured, Female, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells, Liver, Mice, Mice, Inbred C57BL, Mice, Inbred NOD, Mice, SCID, Polymerase Chain Reaction, Pregnancy, Proto-Oncogene Proteins c-kit, Signal Transduction, Wnt Proteins, Wnt3 Protein, Wnt3A Protein

Volume

28

Issue

8

First Page

1379

Last Page

1389

ISSN

1549-4918

JAX Source

Stem Cells 2010 Aug; 28(8):1379-89.

PMID

20521329

Abstract

Quiescent cells lacking expression of mature lineage makers and the c-Kit receptor reside in adult bone marrow. Despite their phenotypic similarity to hematopoietic stem cells, these Lin(-)Sca-1(+)c-Kit(-) cells lack myeloid and erythroid potential and long-term hematopoietic repopulating capacity, whereas, recent studies have functionally demonstrated that the Lin(-)Sca-1(+)c-Kit(-) population contains early lymphoid-committed progenitors. Examining the role of Wnt signaling in regulation of this population, we found that c-Kit(-) cells express diverse Wnt receptors and proliferate upon Wnt pathway activation in vitro and in vivo. Stimulation with Wnt3a, but not Wnt5a or Wnt11, promoted c-Kit(-) cells to give rise to myeloid and erythroid progenitors with robust self-renewal capacity measured by clonal replating. In addition, Wnt3a-stimulated c-Kit(-) cells gave rise to all hematopoietic lineages (lymphoid, myeloid, and erythroid) upon transplant into the liver of newborn recipient mice. Our study reveals that Wnt3a activates unique cell fate decisions of dormant c-Kit(-) that promotes short-term multilineage reconstitution capacity in vivo, thereby revealing a unique role for Wnt activation in hematopoiesis. Overall, our results highlight the potential of utilizing signaling molecules known to have instructive roles in regeneration to discover cell subsets residing in adult organisms with unexploited regenerative capacity.

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