Prdm9 controls activation of mammalian recombination hotspots.

Document Type

Article

Publication Date

2010

Keywords

Amino-Acid-Sequence, Animals, Chromosome-Mapping, Histone-Lysine-N-Methyltransferase, Meiosis, Mice-Inbred-C57BL, Molecular-Sequence-Data, Recombination-Genetic, Sequence-Analysis-DNA, Testis, Zinc-Fingers

First Page

835

Last Page

835

JAX Source

Science 2010; 327(5967):835.

Abstract

Mammalian meiotic recombination, which preferentially occurs at specialized sites called hotspots, ensures the orderly segregation of meiotic chromosomes and creates genetic variation among offspring. A locus on mouse chromosome 17, which controls activation of recombination at multiple distant hotspots, has been mapped within a 181-kilobase interval, three of whose genes can be eliminated as candidates. The remaining gene, Prdm9, codes for a zinc finger containing histone H3K4 trimethylase that is expressed in early meiosis and whose deficiency results in sterility in both sexes. Mus musculus exhibits five alleles of Prdm9; human populations exhibit two predominant alleles and multiple minor alleles. The identification of Prdm9 as a protein regulating mammalian recombination hotspots initiates molecular studies of this important biological control system.

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