Synapse Loss and Dendrite Remodeling in a Mouse Model of Glaucoma.
Document Type
Article
Publication Date
12-4-2015
JAX Source
PLoS One 2015 Dec 4; 10(12):e0144341.
Volume
10
Issue
12
First Page
0144341
Last Page
0144341
ISSN
1932-6203
PMID
26637126
Grant
EY021525
Abstract
It has been hypothesized that synaptic pruning precedes retinal ganglion cell degeneration in glaucoma, causing early dysfunction to retinal ganglion cells. To begin to assess this, we studied the excitatory synaptic inputs to individual ganglion cells in normal mouse retinas and in retinas with ganglion cell degeneration from glaucoma (DBA/2J), or following an optic nerve crush. Excitatory synapses were labeled by AAV2-mediated transfection of ganglion cells with PSD-95-GFP. After both insults the linear density of synaptic inputs to ganglion cells decreased. In parallel, the dendritic arbors lost complexity. We did not observe any cells that had lost dendritic synaptic input while preserving a normal or near-normal morphology. Within the temporal limits of these observations, dendritic remodeling and synapse pruning thus appear to occur near-simultaneously. PLoS One 2015 Dec 4; 10(12):e0144341.
Recommended Citation
Berry R,
Qu J,
John S,
Howell GR,
Jakobs T.
Synapse Loss and Dendrite Remodeling in a Mouse Model of Glaucoma. PLoS One 2015 Dec 4; 10(12):e0144341.