Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing.

Authors

Xin Chen, The Jackson LaboratoryFollow
Lina Kozhaya, The Jackson LaboratoryFollow
Cihan Tastan
Lindsey Placek, The Jackson LaboratoryFollow
Mikail Dogan, The Jackson LaboratoryFollow
Meghan Horne
Rebecca Abblett, The Jackson LaboratoryFollow
Ece Karhan, The Jackson LaboratoryFollow
Martin Vaeth
Stefan Feske
Derya Unutmaz, The Jackson LaboratoryFollow

Document Type

Article

Publication Date

9-1-2018

JAX Source

J Immunol 2018 Sep 1; 201(5):1586-1598

Volume

201

Issue

5

First Page

1586

Last Page

1598

ISSN

1550-6606

PMID

30021769

DOI

https://doi.org/10.4049/jimmunol.1701616

Grant

AI121920, NS105539

Abstract

Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Toward this goal, we used lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guide RNAs that targeted either coding or noncoding regions of genes of interest. We showed that multiple genes (

Recommended Citation

Chen X, Kozhaya L, Tastan C, Placek L, Dogan M, Horne M, Abblett R, Karhan E, Vaeth M, Feske S, Unutmaz D. Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing. J Immunol 2018 Sep 1; 201(5):1586-1598