Deletion of GARP on mouse regulatory T cells is not sufficient to inhibit the growth of transplanted tumors.

Document Type

Article

Publication Date

10-2018

JAX Source

Cell Immunol 2018 Oct; 332:129-133

Volume

332

First Page

129

Last Page

133

ISSN

1090-2163

PMID

30093071

DOI

https://doi.org/10.1016/j.cellimm.2018.07.011

Abstract

GARP is a transmembrane protein that presents latent TGF-β1 on the surface of regulatory T cells (Tregs). Neutralizing anti-GARP monoclonal antibodies that prevent the release of active TGF-β1, inhibit the immunosuppressive activity of human Tregs in vivo. In this study, we investigated the contribution of GARP on mouse Tregs to immunosuppression in experimental tumors. Unexpectedly, Foxp3 conditional garp knockout (KO) mice challenged orthotopically with GL261 tumor cells or subcutaneously with MC38 colon carcinoma cells did not show prolonged survival or delayed tumor growth. Also, the suppressive function of KO Tregs was similar to that of wild type Tregs in the T cell transfer model in allogeneic, immunodeficient mice. In conclusion, garp deletion in mouse Tregs is not sufficient to impair their immunosuppressive activity in vivo.

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