Identification of genetic elements in metabolism by high-throughput mouse phenotyping.
Document Type
Article
Publication Date
1-18-2018
JAX Source
Nat Commun 2018 Jan 18; 9(1):288.
Volume
9
Issue
1
First Page
288
Last Page
288
ISSN
2041-1723
PMID
29348434
DOI
https://doi.org/10.1038/s41467-017-01995-2
Abstract
Metabolic diseases are a worldwide problem but the underlying genetic factors and their relevance to metabolic disease remain incompletely understood. Genome-wide research is needed to characterize so-far unannotated mammalian metabolic genes. Here, we generate and analyze metabolic phenotypic data of 2016 knockout mouse strains under the aegis of the International Mouse Phenotyping Consortium (IMPC) and find 974 gene knockouts with strong metabolic phenotypes. 429 of those had no previous link to metabolism and 51 genes remain functionally completely unannotated. We compared human orthologues of these uncharacterized genes in five GWAS consortia and indeed 23 candidate genes are associated with metabolic disease. We further identify common regulatory elements in promoters of candidate genes. As each regulatory element is composed of several transcription factor binding sites, our data reveal an extensive metabolic phenotype-associated network of co-regulated genes. Our systematic mouse phenotype analysis thus paves the way for full functional annotation of the genome. Nat Commun 2018 Jan 18; 9(1):288.
Recommended Citation
Rozman J,
Rathkolb B,
Oestereicher M,
Schütt C,
Ravindranath A,
Leuchtenberger S,
Sharma S,
Kistler M,
Willershäuser M,
Brommage R,
Meehan T,
Mason J,
Haselimashhadi H,
Hough T,
Mallon A,
Wells S,
Santos L,
Lelliott C,
White J,
Sorg T,
Champy M,
Bower L,
Reynolds C,
Flenniken A,
Murray SA,
Nutter L,
Svenson KL,
West D,
Tocchini-Valentini G,
Beaudet A,
Bosch F,
Braun R,
Dobbie M,
Gao X,
Herault Y,
Moshiri A,
Moore B,
Kent Lloyd K,
McKerlie C,
Masuya H,
Tanaka N,
Flicek P,
Parkinson H,
Sedlacek R,
Seong J,
Wang C,
Moore M,
Brown S,
Tschöp M,
Wurst W,
Klingenspor M,
Wolf E,
Beckers J,
Machicao F,
Peter A,
Staiger H,
Häring H,
Grallert H,
Campillos M,
Maier H,
Fuchs H,
Gailus-Durner V,
Werner T,
Hrabe de Angelis M.
Identification of genetic elements in metabolism by high-throughput mouse phenotyping. Nat Commun 2018 Jan 18; 9(1):288.