An important role for periplasmic storage in Pseudomonas aeruginosa copper homeostasis revealed by a combined experimental and computational modeling study.
Document Type
Article
Publication Date
11-1-2018
Keywords
JGM
JAX Source
Mol Microbiol 2018 Nov; 110(3):357-369
Volume
110
Issue
3
First Page
357
Last Page
369
ISSN
1365-2958
PMID
30047562
DOI
https://doi.org/10.1111/mmi.14086
Abstract
Biological systems require precise copper homeostasis enabling metallation of cuproproteins while preventing metal toxicity. In bacteria, sensing, transport, and storage molecules act in coordination to fulfill these roles. However, there is not yet a kinetic schema explaining the system integration. Here, we report a model emerging from experimental and computational approaches that describes the dynamics of copper distribution in Pseudomonas aeruginosa. Based on copper uptake experiments, a minimal kinetic model describes well the copper distribution in the wild-type bacteria but is unable to explain the behavior of the mutant strain lacking CopA1, a key Cu
Recommended Citation
Parmar J,
Quintana J,
Ramírez D,
Laubenbacher R,
Argüello J,
Mendes P.
An important role for periplasmic storage in Pseudomonas aeruginosa copper homeostasis revealed by a combined experimental and computational modeling study. Mol Microbiol 2018 Nov; 110(3):357-369